Obtain five test tubes and put a mark 2 cm from the bottom of each using a wax pencil. ![]() For example: "The milk in tube #1 will."Ī2. The word "milk" should be used in your hypothesis. Write a hypothesis for this experiment in your lab notebook. ![]() You will test the hypotheses by performing the experiment below.Ī1. An incorrect hypothesis is perfectly acceptable. At this point, you do not know what will happen your hypotheses may be correct or incorrect. Your hypotheses should be specific for this experiment it should state the expected outcome of this experiment.īe sure to write your hypotheses as statements, not as questions. You should have two hypotheses- one that addresses the the effect of temperature on rate of reaction (tubes 1 and 3) and one that addresses the effect of denaturing the enzyme on reaction time (tubes 3 and 5). The word "milk" should appear in each of your hypotheses. Predict what will happen in each of the tubes described in the two experiments described below and write your two hypotheses in your notebook. The presence of a reaction is indicated by milk becoming solid.Ī1. You will observe the activity of this enzyme by mixing it with milk in a test tube. Rennin is an enzyme found in the stomach of mammals where it functions to solidify milk. It will also explore the rate of enzyme activity using an enzyme that has been denatured. This part of the exercise will explore the rate of enzyme activity at two different temperatures. The rate of chemical reactions therefore increases with temperature but then decreases as enzymes denature. Above a certain temperature, activity begins to decline because the enzyme begins to denature. ![]() More collisions increase the likelihood that substrate will collide with the active site of the enzyme, thus increasing the rate of an enzyme-catalyzed reaction. Higher temperature generally causes more collisions among the molecules and therefore increases the rate of a reaction.
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